BIOQUANT OSTEO Imaging Options

BIOQUANT OSTEO collects data on images of tissue slides, welled plates, or anything else that can to captured with the camera or scanned into an image.

The decision of how to collect images in your laboratory is important. There are several imaging techniques for the analysis of bone tissues and cell cultures. The best technique for you lab to use will depend on many factors including available laboratory equipment, the number of people that need to collect data, and the available budget.

Option 1: Open Image Files

Open existing TIF, BMP, PNG, JPG, or DICOM files

This option is ideal for laboratories that have access to existing image equipment, such as a scientific grade digital camera or slide scanner. For this feature set, you only need to purchase BIOQUANT OSTEO.

  • When you open a high resolution TIF or BMP scan from a slide scanner, it loads into our Large Image Navigator. The Large Image Navigator is like a virtual microscope. Read More
     
  • When you open a montage or stitched image from your camera software, it loads into our Large Image Navigator. The Large Image Navigator is like a virtual microscope. Read More
     
  • When you load a series of standard sized TIF, BMP, PNG, or JPG files captured from your digital camera, they load into the Imaging Toolkit. The Imaging Toolkit can sequence through images, loading them one by one into the Measurement window for analysis. Open up to 1,000 standard digital camera images at a time.
     
  • When you load a DICOM 3D image stack, it loads into the DICOM Navigator.
    Using the calibration data in the DICOM file, BIOQUANT calibrates automatically for morphometric measurements. Read More.
     
  • When you load a DICOM 2D X-ray image, it loads into the Large Image Navigator.
    BIOQUANT supports both DICOM and monochrome TIFF formats for x-ray data. This gives you a hardware independent tool for your data analysis.  Read More.
     
  • Of course you can open a single standard sized digital TIF, BMP, PNG, JPG, or DICOM image as well. Standard sized images that were captured with a digital or analog camera open directly into the Measurement window.

Option 2: Live Imaging from Microscope

Get live Image from the microscope Directly in the Measurement Window

This option is ideal of laboratories that have a microscope and one of our supported QImaging cameras. Optionally you can upgrade your stage with a tracking stage, such as stage encoders or a motorized stage. For this feature set, need to purchase the BIOQUANT OSTEO software for musculoskeletal research, plus one if the microscope upgrade kits mentioned below.

A Supported QImaging Camera Enables

  • A live image is shown directly in the Measurement window, not through a 3rd party TWAIN interface. 
  • Fast live imaging at 30 frames per second.
  • Dynamic adjustment of Auto Exposure, Gain, and Background Correction within the Measurement window.
  • Direct measurement on the live image or click the "Held" button to capture the image for automated analysis. 
  • Enhanced Live Redraw:
    Redraw measurement tracings on a live image.
    Redraw stereology grids on a live image.
    Redraw measurement tracings on a live or held image across multiple stage fields of view. Image alignment is necessary.
  • Montage creation using the Imaging Toolkit to capture sequential images and montage them into a single scan. Manual stage movement is necessary. Image alignment is necessary.
  • This option is enabled by purchasing Kit 1, which includes the QImaging BIOQUANT PV1 Camera and a microscope adapter. It requires an existing microscope with a trinocular head. See Microscope Upgrade Kits.

Stage encoders enable

  • Freedom of movement. When you move the stage, its position is known by BIOQUANT. 
  • Field to Field Redraw: Redraw measurement tracings on a live or held image at any magnifications or stage position without manually letting BIOQUANT know that you moved.
    For example, you can define the Tissue Volume at 2x and then collect osteoid data at 10x or 20x. BIOQUANT knows where you are at all mags and stage positions. It redraws the Tissue Volume and measurement tracings correctly.
  • Montage creation using the Imaging Toolkit to capture sequential images and montage them into a single scan. Manual stage movement is necessary. Image alignment is not necessary.
  • This option is enabled by purchasing Kit 2, which includes the QImaging BIOQUANT PV1 Camera, microscope adapter, and stage encoders. It requires an existing microscope with a trinocular head. See Microscope Upgrade Kits.

a motorized stage enables

  • Freedom of movement. When you move the stage, its position is known by BIOQUANT. 
  • Field to Field Redraw: Redraw measurement tracings on a live or held image at any magnifications or stage position without manually letting BIOQUANT know that you moved.
    For example, you can define the Tissue Volume at 2x and then collect osteoid data at 10x or 20x. BIOQUANT knows where you are at all mags and stage positions. It redraws the Tissue Volume and measurement tracings correctly.
  • Montage creation using the Imaging Toolkit to capture sequential images and montage them into a single scan. Manual stage movement is necessary. Image alignment is not necessary.
  • This option is enabled by purchasing Kit 3 or 4, which include the QImaging BIOQUANT PV1 Camera, microscope adapter, and a motorized stage. They require an existing microscope with a trinocular head. See Microscope Upgrade Kits.
  • If you have BIOQUANT SCAN, you also enable all of the features under option 3.

Option 3: Use BIOQUANT Scan to Digitize Slide

Use BIOQUANT SCAN to create a high resolution digital scan of your entire slide

This option is ideal of laboratories that have initial startup funds or are applying for a grant.  For this feature set, you would purchase the BIOQUANT OSTEO musculoskeletal research software, the BIOQUANT SCAN add-on, and either Kit 3 or Kit 4 (see Microscope Upgrade Kits) OR the BIOQUANT Osteoimager Scanning Scope.

  • The BIOQUANT SCAN Add-on supports bright field, polarized light, and fluorescence slide scanning. 
  • Scan up to 8 tissue sections on 4 slides. The typical scan time is about 8 minutes per section with a 20X objective.
  • Scan up to 96 wells per plate, at a typical scan time of about 3 minutes per well with a 10X objective.
  • Scans can be saved as large TIF images or in the BIOQUANT Image File (BIF) format. The BIF format stores calibration information and be opened on a BIOQUANT OSTEO Satellite system for analysis, freeing up the core BIOQUANT OSTEO system.
  • Scans loads into our Large Image Navigator. The Large Image Navigator is like a virtual microscope. Read More.
  • A microscope scanning kit upgrade or the BIOQUANT Osteoimager Scanning Scope is required. 
  • Read More about BIOQUANT SCAN.