Implant Osseointegration Protocol

Sectioning and Staining

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Osseointegration normally relies on ground sections because it’s generally not possible to cut metal implants on a microtome. Surface staining methods are therefore required. 

Bone Formation Boundary

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A standard technique in osseointegration is the analysis of new bone formation and bone contact surface within a standard zone around the implant. BIOQUANT includes tools to standardize the thickness of the sampling area around the implant. A zone thickness is specified and then a drawing tool guides a technician in tracing out the sampling area. In the image at the top, a zone thickness of 125 microns was used.

Referent Data

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Once the sampling area is defined, BIOQUANT automatically collects three pieces of referent data used to normalized the other values measured on the section:

  • Im.Ar (Implant Area)

  • Im.Pm (Implant Perimeter)

  • Sa.Ar (Sampling Area)

FYI - The Referent Data Tool can be used on a large scan of the tissue, zoomed out to fit in the Image window as show above. It can also be used field-to-field at higher magnification. You do not need a scan or tracking device to use BIOQUANT, although they do save you time.

Bone Structure

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Referent Data Tool

Bone in the sampling is identified by specific staining and color-based thresholding tools. Draw and Erase tools allow corrections to be made to the automatic threshold. Once the bone is defined, BIOQUANT measures:

  • B.Ar, B.Pm

  • Tb.N, Tb.Sp, Tb.Dm

Bone Contact Surface 

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The portion of the implant surface that is occupied by bone is marked at high magnification. BIOQUANT then computes:

  • Co.Pm

  • Co.Pm/B.Pm

  • Co.Pm/Im.Pm

Repeat for Remaining Implant Surface

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Recent Citations of BIOQUANT in Implant Research

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