Three dimensional printed macroporous polylactic acid/hydroxyapatite composite scaffolds for promoting bone formation in a critical-size rat calvarial defect model


Haifeng Zhang, Xiyuan Mao, Zijing Du, Wenbo Jiang, Xiuguo Han, Danyang Zhao, Dong Han & Qingfeng Li


We have explored the applicability of printed scaffold by comparing osteogenic ability and biodegradation property of three resorbable biomaterials. A polylactic acid/hydroxyapatite (PLA/HA) composite with a pore size of 500 μm and 60% porosity was fabricated by three-dimensional printing.3D printed PLA/HA,

β-tricalcium phosphate (β-TCP) and partially demineralized bone matrix (DBM) seeded with bone marrow stromal cells (BMSCs) were evaluated by cell adhesion, proliferation, alkaline phosphatase activity and osteogenic gene expression of osteopontin (OPN) and collagen type I (COL-1).Moreover, the biocompatibility,

bone repairing capacity and degradation in three different bone substitute materials were estimated using a critical-size rat calvarial defect model in vivo. The defects were evaluated by micro-computed tomography and histological analysis at 4 and 8weeks after surgery, respectively. The results showed that each of the studied scaffolds own its specific merits and drawbacks. 3D printed PLA/HA scaffolds possessed good biocompatibility and stimulated BMSCs cell proliferation and differentiation to osteogenic cells. And the outcomes in vivo revealed that 3D printed PLA/HA scaffolds had good osteogenic capability and biodegradation activity with no difference inflammation reaction. Therefore, 3D printed PLA/HA scaffolds have the potential applications in bone tissue engineering and may be used as graft substitutes in reconstructive surgery

Link to Article