Estimation with Stereology
Stereology is the use of statistical sampling to estimate the morphology of structures. It is particularly helpful when limited stain contrast makes automated analysis difficult.
Increases Efficiency Relative to Manual Tracing
Calibrated Image Montage
Calibrated Image Montage puts a single image of the entire sampling area (e.g. tissue volume) in the Image window, making it simple to accurately draw the sampling area boundary. The BIOQUANT Imaging Toolkit Plug-in is required for this feature.
The Grid Wizard automatically generates sampling grids bounded by the Tissue Volume. Grids automatically re-size with changes in zoom level on the digital scan, or magnification if using live imaging.
- Count intersections between the bone surface and the grid lines to estimate bone surface in trabecular bone.
- Count intersections between the bone and the points within the grid to estimate bone volume
Grids OVER Digital Scans
Grids can be superimposed over digital scans.
Grids Over a Live Image Using Motorized Stages
Grids can be superimposed over the live image from an attached digital camera. As the stage moves, the grid is moved along with the tissue.