BIOQUANT OSTEO
Arthritis Protocol


Imaging and Staining

The guinea pig is common non-surgical model for arthritis. Since the guinea pig tibia is quite
large, scanning is a helpful solution. This can be done either with a scanning upgrade to your lab’s microscope or in an imaging core with a digital pathology scanner.

Epiphyseal Bone Boundary

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ROI Tools

BIOQUANT has several unique tools to help you define tissue volumes quickly and consistently. The Irregular TV tool makes it easy to define the outer “epiphyseal tissue volume” containing all the bone distal to the articular cartilage and proximal of the growth plate, including the cortical shell.

Irregular TV tool makes it easy to define the outer “epiphyseal tissue volume” containing all the bone distal to the articular cartilage and proximal of the growth plate, including the cortical shell

Epiphyseal Bone Structure

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Referent Data tool 

With a single click, the Referent Data tool identifies and thresholds bone within the epiphyseal tissue volume, and automatically fills small holes and cracks while preserving actual voids in the bone. The Referent Data tool collects these data simultaneously:

  • Ep.TV, Ep.BV, Ep.BS
  • Ep.BV/Ep.TV, Ep.BS/Ep.BV
  • Ep.Tb.N, Ep.Tb.Dm, Ep.Tb.Sp.

Trabecular Bone Boundary

ROI TOOLS

BIOQUANT has several unique tools to help you define tissue volumes quickly and consistently. The Irregular TV tool makes it easy to define the outer “epiphyseal tissue volume” containing all the bone distal to the articular cartilage and proximal of the growth plate, including the cortical shell.

Irregular TV tool makes it easy to define the outer “epiphyseal tissue volume” containing all the bone distal to the articular cartilage and proximal of the growth plate, including the cortical shell

Trabecular Bone Structure

Referent Data tool

Second, a “trabecular tissue volume” is drawn that contains only epiphyseal trabecular bone, excluding the cortical shell, subchondral bone plate, and bone proximal to the growth plate.
Trabecular structure analysis

Next, BIOQUANT will re-use the threshold identification of the bone made in the previous
step. With the bone structure now restricted to the trabecular compartment, BIOQUANT collects these data simultaneously:

  • TV, BV, BS
  • BV/TV, BS/BV
  • Tb.N, Tb.Dm, Tb.Sp.  

Subchondral Bone Plate Structure

Click image to enlarge.

Subchondral plate definition

Using the epiphyseal tissue volume as a boundary, BIOQUANT again retrieves the bone definition. 
With the Keep tool, you mark the subchondral plate. BIOQUANT removes the rest of the trabecular bone structure. Using the Auto Width tool, BIOQUANT collects these data simultaneously: 

  • Plate area 
  • Bone-cartilage interface length 
  • Plate thickness every 50 microns